Patterned Optogenetic Stimulation Using a DMD Projector.
| Title | Patterned Optogenetic Stimulation Using a DMD Projector. |
| Publication Type | Book Chapter |
| Year of Publication | 2021 |
| Authors | Bhatia A, Moza S, Bhalla US |
| Book Title | Channelrhodopsin |
| Volume | 2191 |
| Series Volume | Methods Mol Biol |
| Pagination | 173-188 |
| Publisher | Humana-Springer |
| ISBN Number | 978-1-0716-0829-6 |
| Abstract | Optical manipulation is a powerful way to control neural activity in vitro and in vivo with millisecond precision. Patterning of light provides the remarkable ability to simultaneously target spatially segregated neurons from a population. Commercially available projectors provide one of the simplest and most economical ways of achieving spatial light modulation at millisecond timescales. Here, we describe the protocol for constructing a projector-based spatio-temporal light patterning system integrated with a microscope on a typical electrophysiology rig. The set-up is well suited for applications requiring rapid, distinct, and combinatorial inputs, akin to brain activity. This equipment involved is fairly economical (<$5000 including all optical and mechanical components), and the set-up is easy to assemble and program. |
| DOI | 10.1007/978-1-0716-0830-2_11 |
| Abbreviation | Methods Mol. Biol. |
| PubMed ID | 32865745 |