Course Schedule 2023
COURSE SCHEDULE
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Sunday, Sept 24th : Opening Lecture, Poster Session
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12:30-13:30 |
Lunch Location: NCBS Canteen First floor |
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13:30-14:00 |
Participants Registration Location: Reception, Southern Laboratories Complex (SLC) |
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14:00-14:30 |
Opening Remarks: Satyajit Mayor Location: Dasheri (SLC) |
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14:30 – 15:30 |
Research Lecture: Jan Ellenberg, EMBL Heidelberg Location: Dasheri |
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15:30-16:00 |
Coffee Break Location: Outside Raspuri |
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16:00-17:00 |
Didactic Lecture 1: Basics of Microscopy, Kohler illumination, Diffraction, Resolution, and Aberrations – Jennifer Ross, Syracuse University, USA Location: Raspuri |
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17:00-17:15 |
Group Photo |
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17:15-20:00 |
Poster Session by the students attending the course Location: Collonade, SLC |
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20:00 – 21:00 |
Dinner Location: NCBS Canteen 1st Floor |
Monday, Sept 25th : Basics of microscopy, Microscope Light path, Diffraction,Resolution, Phase and Polarization
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8:30-13:15 |
Laboratory 1: Microscopy and Kohler Illumination on rails Lab Leader: Jennifer Ross Lab Instructors: Rahul Roy, Satyajit Mayor, Thomas Van Zanten, Luke Lavis, Thorsten Wohland, Mahipal Ganji Lab Support: Raman S, Nayan Chakraborty, Ajay Kumar, Piyush Chhajed, Abrar Rizwi, Sahnawaz Molla, Sunil Kumar (Zeiss), Yathish CL (Zeiss) Location: Raspuri |
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11:0-11:15 |
Coffee Break Location: Outside Raspuri |
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13:15 -14:15 |
Lunch Location: inStem Canteen 1st Floor |
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14:15 – 15:15 |
Didactic Lecture 2: Contrast Enhancement – Phase contrast, Dark Field, Polarized Light, DIC – Jennifer Ross Location: Raspuri |
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15:15-16:15 |
Didactic Lecture 3: Principles of Fluorescence microscopy (Fluorescent dyes and proteins, and selection of Fluorescent Probes) – Luke Lavis Location: Raspuri |
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16:15-16:30 |
Coffee Break Location: Outside Raspuri |
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16:30-18:30 |
Laboratory 2: Phase contrast, DIC and Dark Field Microscopy Lab Leader: Jennifer Ross Lab Instructors: Rahul Roy, Satyajit Mayor, Thomas Van Zanten, Luke Lavis, Thorsten Wohland, Minhaj Sirajuddin Lab Support: Raman S, Nayan Chakraborty, Ajay Kumar, Piyush Chhajed, Abrar Rizwi, Sahnawaz Molla, Sunil Kumar (Zeiss), Yathish CL (Zeiss) Location: Raspuri |
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18:30 – 19:30 |
Didactic Lecture 4: Cameras and Detectors – Rahul Roy Location: Raspuri |
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19:30 – 20:30 |
Dinner Location: NCBS Canteen 1st Floor |
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20:30 – 23:00 |
Free time on Microscopes |
Tuesday, Sept 26th : Fluorescence Microscopy
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8:30-10:30 |
Laboratory 3: Fluorescence Lab A: Examine the fluorescence light path and dichroic mirrors/ filters Lab Leader: Luke Lavis Lab Instructors: Minhaj Sirajuddin, Jennifer Ross, Rahul Roy, Darius Koester, Thomas Van Zanten, Mahipal Ganji, and Company Application Specialists Lab Support: Neelanjana Ray, Swarnadip Ghosh, Shah E-Jahan, Nivya Mendon, Anubhav Prakash, Piyush Chhajed Location: Raspuri |
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10:30-10:45 |
Coffee Break |
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10:45 -11:45 |
Didactic Lecture 5: TIRF Microscopy – Minhaj Sirajuddin Location: Raspuri |
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11:45 – 12:45 |
Didactic Lecture 6: Image Analysis –Christian Tischer Location: Raspuri |
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12:45-13:45 |
Lunch Location: inStem Canteen 1st Floor |
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13:45-15:45 |
Laboratory 4: Fluorescence Lab B - Fluorescence, Time Lapse, Cameras and Z Projections of PSF Lab Leader: Rahul Roy Lab Instructors: Luke Lavis, Minhaj Sirajuddin, Satyajit Mayor, Christian Tischer, Mahipal Ganji and Company Application Specialists Lab Support: Neelanjana Ray, Swarnadip Ghosh, Shah E Jahan, Nivya Mendon, Anubhav Prakash, Piyush Chhajed Location: Raspuri |
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15:45 – 16:45 |
Research Lecture 2: Jennifer Ross, Syracuse University USA Location: Dasheri |
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16:45 – 17:00 |
Coffee Break |
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17:00 – 19:00 |
Laboratory 5: Image Processing / Analysis Workshop Lab leader: Christian Tischer Lab Support: Lakshmi B, Sukanta Behera, Swarang Pundlik, Suraj Jagtap Location: Raspuri |
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19:00 – 20:00 |
Free Time on Microscopes |
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18:30 – 21:00 |
Dinner (for Faculty – Down Town Bangalore) |
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20:00 – 21:00 |
Dinner (for Participants – NCBS Canteen First floor) |
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21:00 – 23:00 |
Free time on Microscopes |
Wednesday, Sept 27th: Optical Sectioning and Enhancing Resolution
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8:30-9:30 |
Didactic Lecture 7: FRET Microscopy – Satyajit Mayor Location: Raspuri |
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9:30-10:30 |
Didactic Lecture 8: Optical Sectioning Techniques – Confocal Microscopy – Thomas van Zanten Location: Raspuri |
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10:45 -15:45 |
Laboratory 6: Optical Sectioning Techniques (point scanning, line scanning, spinning disk), 2 x 2hr modules Lab Leader: K Venkatesan Iyer Lab Support: Neelanjana Ray, Swarnadip Ghosh, Shah E Jahan, Nivya Mendon, Anubhav Prakash, Piyush Chhajed Equipment 1: Olympus FV3000 scanning microscope Lab Instructor: Olympus Application Specialist Location: CIFF, SLC
Equipment 2: Olympus FV3000 point scanning microscope Lab Instructor: Olympus Application Specialist Location: CIFF, inStem
Equipment 3: Andor Spinning Disk Microscope Lab Instructor: Greeshma Pradeep Location: Satyajit Mayor Lab, SLC, First Floor
Equipment 4: Zeiss LSM 980 point-scanning microscope Lab Instructor: Zeiss Application Specialist Location: CIFF, SLC
Equipment 5: Olympus Upright point scanning microscope Lab Instructor: Olympus Application Specialist Location: CIFF, SLC |
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12:45 – 13:45 |
Lunch Location: inStem Canteen 1st Floor |
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15:45 – 16:00 |
Coffee Break |
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16:00-17:00 |
Research Lecture 3: Luke Lavis, Janelia Research Campus Location: Dasheri (SLC) |
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17:00-18:00 |
Research Lecture 4: Thorsten Wohland, NUS, Singapore Location: Dasheri (SLC) |
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18:00 – 18:30 |
Zeeshan Khan Memorial Award Presentation |
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18:30 – 19:30 |
Dinner Location: NCBS Canteen First floor |
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19:00 – 22:00 |
Cultural and Social Event |
Thursday, Sept 28th : Specialized Techniques
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8:30-9:30 |
Didactic Lecture 9: Fluorescence Microscopy based methods to study protein dynamics in live cells: from FRAP to FCS – Thorsten Wohland Location: Raspuri |
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9:30-10:30 |
Didactic Lecture 10 : Spatial Biology – Kevin Matthew Byrd Location: Raspuri |
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10:30 -10:45 |
Coffee Break Location: Outside Raspuri |
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10:45 – 14:45 |
Laboratory 8: Specialized techniques 2 x 1.5hr modules, distributed by student signup and selection. There will be 2 rotations of 1.5 hour each. Students can choose in advance and a rotation plan will be prepared. Technique 1 : Homo-Försters Resonance Energy Transfer (Homo-FRET) Equipment: Nikon TIRF microscope with dual camera Lab Leader: Satyajit Mayor Lab Instructor: Sarayu Beri Lab Support: Location: Satyajit Mayor Lab, SLC
Technique 2: Fluorescence Correlation Spectroscopy (FCS) Equipment: Zeiss LSM 980 with Airyscan Lab Instructor: Thorsten Wohland Lab Support: Sankarshan Talluri, Bhagyashree Mahajan, Suraj K Location: CIFF, SLC
Technique 3: Spatial discovery of Lineage specific markers Equipment: to be decided Lab leader: Kevin Matthew Byrd Lab Instructor: Arjun Guha Lab Support: Neenu Joy, Ibrahim, Shahnawaz Molla Location: tba
Technique 4: Total Internal Reflection Fluorescence (TIRF) Microscopy Equipment: TIRF microscope Lab Leader: Minhaj Sirajuddin Lab Instructor: Rayees Ganie Lab Support: Greeshma Pradeep Location: Minhaj Lab, inStem 1st Floor
Technique 5: STED Super Resolution Microscopy Equipment: Abberior STED Microscope Lab Leader: Thomas van Zanten Lab Instructor: Venkatesan Iyer Lab Support: Raksha K, Gaurav Singh, Suraj RC
Technique 6: STORM Super Resolution Microscopy Equipment: Nikon NSIM/NSTORM Microscope Lab Leader: Mahipal Ganji Lab Instructor: Nikon Application Specialist Lab Support: Sowmya Jahnvi, Aditya Upasani Location: CIFF, SLC
Technique 7: Lightsheet Imaging Equipment: Zeiss Lightsheet 7 microscope Lab leader: Zeiss Application specialist Lab Support: Shivangi Pandey, Drisya Dileep |
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12:15 – 13:15 |
Lunch Location: inStem Canteen 1st Floor |
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14:45- 15:00 |
Coffee Break Location: Outside Raspuri |
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15:00 – 17:00 |
Technical Presentations Location: Dasheri |
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17:00 – 17:15 |
Coffee Break Location: Outside Raspuri |
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17:15– 18:15 |
Didactic Lecture 11: to be decided |
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18:15 – 19:30 |
Free Time on Microscopes |
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19:30 – 21:00 |
Dinner Location: NCBS Canteen First floor |
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20:30 – 22:00 |
Free time on Microscopes/Camera lab |
Friday, Sept 29th : Research Lectures, Specialized Techniques
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8:30-9:30 |
Didactic Lecture 12: Super-resolution Microscopy – STEM and SIM – Thomas van Zanten Location: Raspuri |
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9:30-10:30 |
Didactic Lecture 13 : Super-resolution Microscopy – PALM/STORM - Mahipal Ganji Location: Raspuri |
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10:30 -10:45 |
Coffee Break |
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10:45 – 14:45 |
Laboratory 8: Specialized techniques 2 x 1.5hr modules, distributed by student signup and selection. There will be 2 rotations of 1.5 hour each. Students can choose in advance and a rotation plan will be prepared. Technique 1 : Homo-Försters Resonance Energy Transfer (Homo-FRET) Equipment: Nikon TIRF microscope with dual camera Lab Leader: Satyajit Mayor Lab Instructor: Sarayu Beri Lab Support: Location: Satyajit Mayor Lab, SLC
Technique 2: Fluorescence Correlation Spectroscopy (FCS) Equipment: Zeiss LSM 980 with Airyscan Lab Instructor: Thorsten Wohland Lab Support: Sankarshan Talluri, Bhagyashree Mahajan, Suraj K Location: CIFF, SLC
Technique 3: Spatial discovery of Lineage specific markers Equipment: to be decided Lab leader: Kevin Matthew Byrd Lab Instructor: Arjun Guha Lab Support: Neenu Joy, Ibrahim, Shahnawaz Molla Location: tba
Technique 4: Total Internal Reflection Fluorescence (TIRF) Microscopy Equipment: TIRF microscope Lab Leader: Minhaj Sirajuddin Lab Instructor: Rayees Ganie Lab Support: Greeshma Pradeep Location: Minhaj Lab, inStem 1st Floor
Technique 5: STED Super Resolution Microscopy Equipment: Abberior STED Microscope Lab Leader: Thomas van Zanten Lab Instructor: Venkatesan Iyer Lab Support: Raksha K, Gaurav Singh, Suraj RC
Technique 6: STORM Super Resolution Microscopy Equipment: Nikon NSIM/NSTORM Microscope Lab Leader: Mahipal Ganji Lab Instructor: Nikon Application Specialist Lab Support: Sowmya Jahnvi, Aditya Upasani Location: CIFF, SLC Technique 7: Lightsheet Imaging Equipment: Zeiss Lightsheet 7 microscope Lab leader: Zeiss Application specialist Lab Support: Shivangi Pandey, Drisya Dileep |
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12:15 – 12:30 |
Group Photo |
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12:30 – 14:15 |
Discussion over Lunch: Small group discussions of students with faculty over lunch about their research/ microscopy interests Location: inStem Canteen 1st Floor |
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16:00-17:00 |
Research Lecture 5: Satyajit Mayor, NCBS, Bangalore Location: Dasheri (SLC) |
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17:00-18:00 |
Research Lecture 6: Kevin Matthew Byrd, UNC-CH and ADASRI, USA Location: Dasheri (SLC) |
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18:00 – 18:30 |
Technical Lecture Location: Dasheri (SLC) |
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18:30 – 19:30 |
Graduation |
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19:30 – 21:00 |
Dinner Location: NCBS Canteen 1st Floor |
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21:00 – 23:00 |
Project briefing/Free Time on Microscopes |
Saturday, Sept 30th: Projects
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9:00-10:00 |
Research Lecture 7: Rahul Roy, Indian Institute of Science, Bangalore, India Location: Dasheri (SLC) |
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10:00-11:00 |
Research lecture 8: Vinothkumar Kutti Raghunath, NCBS, Bangalore, India Location: Dasheri (SLC) |
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11:00 -11:15 |
Coffee Break Location: Outside Raspuri |
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11:15 – 13:00 |
Projects |
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13:00 – 14:00 |
Lunch Location: NCBS Canteen 1st Floor |
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14:00 – 16:00 |
Projects |
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16:00-16:15 |
Coffee Break Location: Outside Raspuri |
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16:15 – 18:15 |
Projects |
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18:15 – 22:00 |
Workshop Dinner and Social (Downtown Bangalore) |
Sunday, Oct 1st : Projects
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9:00-11:00 |
Projects |
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11:00-11:15 |
Coffee Break Location: Outside Raspuri |
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11:15 – 13:00 |
Projects |
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13:00 – 14:00 |
Lunch Location: NCBS Canteen 1st Floor |
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14:00 – 16:00 |
Projects |
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16:00 – 16:15 |
Coffee Break Location: Outside Raspuri |
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16:15 – 18:15 |
Project presentation by participants |
