Extraction and Purification of Laccases from Rice Stems.
| Title | Extraction and Purification of Laccases from Rice Stems. |
| Publication Type | Journal Article |
| Year of Publication | 2019 |
| Authors | Swetha C, Shivaprasad PV |
| Journal | Bio Protoc |
| Volume | 9 |
| Issue | 7 |
| Pagination | e3208 |
| Date Published | 2019 Apr 05 |
| ISSN | 2331-8325 |
| Abstract | Laccases are found in cell walls of plants in very low amounts. This protocol provides an efficient method to purify laccases from rice stems. The method involves three steps: 1) Isolation of total protein from rice stems using buffers with high salt concentration to extract protein from cell walls; 2) Purification of laccases using concanavalin-A beads; and, 3) In-gel staining of laccases with 4-hydroxyindole. Concanavalin-A specifically binds to internal or non-reducing terminal α-D-mannosyl and α-D-glucosyl groups found in glycoproteins and glycolipids. Laccases being glycoproteins binds to concanavalin-A during purification process and eluted with mannose. |
| DOI | 10.21769/BioProtoc.3208 |
| Alternate Journal | Bio Protoc |
| PubMed ID | 33655003 |
| PubMed Central ID | PMC7854116 |