Fast, Efficient, and Stable Conjugation of Multiple DNA Strands on Colloidal Quantum Dots.
| Title | Fast, Efficient, and Stable Conjugation of Multiple DNA Strands on Colloidal Quantum Dots. |
| Publication Type | Journal Article |
| Year of Publication | 2015 |
| Authors | Banerjee A, Grazon C, Nadal B, Pons T, Krishnan Y, Dubertret B |
| Journal | Bioconjug Chem |
| Volume | 26 |
| Issue | 8 |
| Pagination | 1582-9 |
| Date Published | 2015 Aug 19 |
| ISSN | 1520-4812 |
| Abstract | A novel method for covalent conjugation of DNA to polymer coated quantum dots (QDs) is investigated in detail. This method is fast and efficient: up to 12 DNA strands can be covalently conjugated per QD in optimized reaction conditions. The QD-DNA conjugates can be purified using size exclusion chromatography and the QDs retain high quantum yield and excellent stability after DNA coupling. We explored single-stranded and double-stranded DNA coupling, as well as various lengths. We show that the DNA coupling is most efficient for short (15 mer) single-stranded DNA. The DNA coupling has been performed on QDs emitting at four different wavelengths, as well as on gold nanoparticles, suggesting that this technique can be generalized to a wide range of nanoparticles. |
| DOI | 10.1021/acs.bioconjchem.5b00221 |
| Alternate Journal | Bioconjug. Chem. |
| PubMed ID | 25992903 |