Optimized protocol for assay for transposase-accessible chromatin by sequencing (ATAC-seq) from Drosophila melanogaster brain tissue.
| Title | Optimized protocol for assay for transposase-accessible chromatin by sequencing (ATAC-seq) from Drosophila melanogaster brain tissue. |
| Publication Type | Journal Article |
| Year of Publication | 2023 |
| Authors | Dhall JKaur, Kasturacharya N, Pandit A, Cp L |
| Journal | STAR Protoc |
| Volume | 4 |
| Issue | 2 |
| Pagination | 102153 |
| Date Published | 2023 Apr 18 |
| ISSN | 2666-1667 |
| Abstract | Transposase-accessible chromatin by sequencing (ATAC-seq) has emerged as an advantageous technique to assess chromatin accessibility owing to the robustness of "tagmentation" process and a relatively faster library preparation. A comprehensive ATAC-seq protocol from Drosophila brain tissue is currently unavailable. Here, we have provided a detailed protocol of ATAC-seq assay from Drosophila brain tissue. Starting from dissection and transposition to amplification of libraries has been elaborated. Furthermore, a robust ATAC-seq analysis pipeline has been presented. The protocol can be easily adapted for other soft tissues. |
| DOI | 10.1016/j.xpro.2023.102153 |
| Alternate Journal | STAR Protoc |
| PubMed ID | 37074906 |
| PubMed Central ID | PMC10148076 |