The course aims to train the EM users in EM tomography techniques enabling them to carry out 3D imaging of biological/material samples in their home institute.
Two methods of acquiring the data will be demonstrated depending on the type of EM being used; TEM or SEM:

  • A (dual-axis) tomographic tilt-series for TEM users
  • Correlative Light and Electron Microscopy (CLEM) with array series for SEM users.

The course lectures will be given by experts in the field supplemented by practicals, covering  all aspects of tomography:

  • Tomography theory
  • Sample preparation for tomo series collection
  • Data collection for Tilt series, Serial tomography and CLEM (SIM and SEM)
  • Alignment
  • Reconstruction
  • Segmentation and Visualization
  • Quantitative Analysis of 3D datasets