Laboratory of Biomolecular Structure, Dynamics and Function

Research Interests

Host-Microbe Interactions

Microbes hijack the host machinery to their own advantage. During infection, several host defense mechanisms are destabilized by the microbes using the ubiquitin-proteasome pathway. We study the interactions between microbial factors and host proteins to understand this process.

Protein Quality Control

Misfolding of proteins can lead to accimulation of toxic aggregates. It is assumed that higher energy intermediates along the protein folding landscape seed the formation of toxic aggregates. Protein quality control systems like the Ubiquitin-proteasome pathway ensures that these potentially toxic species are degraded. We are interested to get insights into the structure of higher energy intermediates. We also investigate how misfolded proteins targeted by the ubiquitin-proteasome pathway.

 

 

 

 

 

 

Positions: We are actively looking for postdocs with a Phd in protein-chemistry/protein-Xray-crystallography/protein-NMR and Virology. Please apply with a detailed CV to rana@ncbs.res.in. Your CV should reflect your expertise and research interest.

Students interested in Phd are requested to apply through the NCBS-PhD program. There are no summer/winter positions available.

Recent Selected Publications

Negi H, Reddy PP, Vengayil V, Patole C, Laxman S, Das R, (2020), A novel polyubiquitin chain linkage formed by viral Ubiquitin is resistant to host deubiquitinating enzymes, Biochem J., BCJ20200289. doi: 10.1042/BCJ20200289.

Habibullah, BI, Tripathi, V, Surana P, Das R, (2020), Monitoring Protein Ubiquitination and SUMOylation in real-time by NMR, Chemical Communications, 2020, 56, 6735 – 6738.

Hembram DSS, Negi H, Biswas P, Tripathi V, Bhushan L, Shet D, Kumar V, Das R, (2020), The viral SUMO-targeted Ubiquitin Ligase ICP0 is phosphorylated and activated by host kinase Chk2, J. Mol. Biol., 432(7):1952-1977.

Mukherjee A, Singh R, Udayan S, Biswas S, Reddy PP, Manmadhan S, George G, Kumar S, Das R, Rao BM, Gulyani A., (2020), A Fyn biosensor reveals pulsatile, spatially localized kinase activity and signaling crosstalk in live mammalian cells, eLife 9: e50571.

Mohanty P, Agrata R, Habibullah BI, Geetha Surendran A, Das R. (2019), Deamidation disrupts native and transient contacts to weaken the interaction between UBC13 and RING-finger E3 ligases, eLife 8: e492238.

Nandwani N, Surana P, Negi H, Mascarenhas NM, Udgaonkar JB*, Das R*, Gosavi S*, (2019), A five-residue motif for the design of domain swapping in proteins, Nature Communications, 10(1):452. *Corresponding authors.

For a complete list of publications, please click here.