Electron Microscopy Facility - MicroCT
X-ray Micro-Computerized Tomography
Micro CT Scanner Configuration:
- Skyscan 1272, Bruker Instruments
- Microfocus sealed X-ray source 20-100kV, 10W, <5um spot size @ 4W.
- X-ray detector 16Mp (4904x3280 pixels) or 11Mp (4032x2688 pixels), 14bit cooled CCD fiber-optically coupled to scintillator
- Pixel size on the object at maximum magnification [nominal resolution] is 0.35um (16Mp) or 0.45um (11Mp)
- Reconstruction arrays – up to 14450x14450x2630pixels (16Mp) or 11840x11840x2150pixels (11Mp) after a single scan
- Maximum scanning object diameter - 1. 75mm (three camera offset positions) 2. 52mm(two camera offset positions) 3. 26mm (central camera position) Fastest Scan
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6-position integrated filter changer, standard filters: no filter, Al 0.25mm, Al 0.5mm, Al 1mm, Al 0.5mm + Cu 0.038mm, Cu 0.11mm
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Integrated micro-positioning stage, 5-7mm travel (dependent on orientation)
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Radiation safety: <1microSv/h in 15cm from any point of instrument surface
Optional sample changer:
- Automatic sample changer with 16 sample positions
- Maximum sample size: 25mm diameter, 50 mm length
- Sample mounts with four sizes, 16pcs of each size
- Scanned samples can be removed / replaced without interrupting scanning process
- Typical sample replacement time is 65 sec.
Control computer:
- DELL T7600 Workstation, Windows 7/64bit:
- Two Intel XEON E5-2687W eight-core 2.3GHz processors
- 128GB 1600MHz RAM
- 12TB (4x3TB on RAID0) HDD
- 2GB NVIDIA graphical card + 6GB NVIDIA Tesla C2075 GPU processor
Bruker Image Processing Software:
- Skyscan -Scanner and sample changer control, image acquisition
- NRecon -Tomography, 3D reconstruction and automatic stitching of oversize scans
- CTVox - Volume rendering, pseudo colouration, animation and movie compilation
- CTVol -3D surface model editing, 3D structure assembly, animation and movie compilation
- CTAn -Semi-automatic segmentation, 2D/3D tissue morphometry and surface model generation
- Dataviewer - 3D orientation editing, 2D/3D image registration and pseudo colouration
Applications:
- Non-invasive visualization, segmentation and morphometry of insect internal anatomy
- Bone density quantification
- Surface area, volume, structure thickness, mineral density and pore network analysis in 2D/3D