1. Ultramicrotomy is a method for cutting specimens into ultra-thin sections, that can be studied and documented at different magnifications in a transmission electron microscope(TEM) or scanning transmission electron microscope(STEM). It is used mostly for biological specimens, but sections of plastics and soft metals can also be prepared. For best resolutions, sections should be from 30 to 70 nm.

2. Cryo ultramicrotomy, is a technique similar to room temperature ultramicrotomy but carried out at freezing temperatures between −70° and −120°C. Cryo ultramicrotomy can be used to cut ultra-thin frozen biological specimens. One of the advantages over the more "traditional" ultramicrotomy process is speed, as it is possible to prepare, freeze and section a specimen faster than resin embedded method.

3. Serial Section on Silicon Wafers for Ultra-Structural Volume Imaging of Cells and Tissues for 3D reconstruction in FESEM, High resolution, three-dimensional (3D) representations of cellular ultra structure are essential for structure function studies in all areas of cell biology. While serial section imaging using transmission electron microscopy can reveal limited subcellular volumes, complete ultra structural reconstructions of large volumes,entire cells or even tissue are difficult to achieve using TEM. We have successfully introduced a new attachment for serial sectioning of tissue for our existing ultramicrotome. Ribbons containing hundreds of 150 nm-200 nm thick sections can be generated, lined on a silicon wafer and imaged serially to obtain 3D ultrastructure of large volumes with high resolution using the combination of imaging techniques in the Zeiss FEG SEM.